IN VITRO PLANTLET REGENERATION OF CHRYSANTHEMUM (Chrysanthemum morifolium)

Abstract

The present research was carried out in Biotechnology Laboratory of the Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka-1207 from the period of February 2016 to June 2017 for in vitro regeneration of chrysanthemum where nodal segments were used as explants. Five levels of BA (1.0, 2.0, 3.0, 4.0 and 5.0 mg/l) and IAA (0.5, 1.0, 1.5, 2.0 and 2.5mg/l) were used for shoot and root induction. 2,4-D (0.5, 1.0, 1.5 and 2.0 mg/l) was used in combination with 2.0 mg/l of BA for callus and shoot induction. The highest frequency of shoot (80.00%) in minimum 7.60 days and maximum number of shoot (2.20, 2.60 and 3.00) at 14, 21 and 28 DAI (days after induction) were recorded from BA 2.0 mg/l. Moreover, the highest length of shoot and number of leaves was noticed from the 3.0 mg/l and 2.0 mg/l of BA, respectively at 14, 21 and 28 DAI. The highest frequency of callus (76.00%) was observed within 14.20 days in MS medium and weight of callus (1.80 g, 2.70 g and 3.50 g) was obtained with BA 2.0 mg/l+ 2,4-D 1.0 mg/l. The maximum number of shoot (3.20) at 40 DAI was obtained with BA 2.0 mg/l+ 2,4-D 1.0 mg/l within minimum 9.20 days. IAA 0.5 mg/l produced the highest percentage of root (80.00%) within 13.00 days while longer days required in other treatments. The maximum number of root (2.60, 3.60 and 5.40) was obtained from 1.5 mg/l IAA at 14, 21 and 28 DAI, respectively. The maximum length of shoot (2.48 cm, 2.84 cm and 3.66 cm) at 14, 21 and 28 DAI was noticed from the BA 2.0 mg/l+ IAA 1.0 mg/l. In combined effect the highest percentage (76%) of root induction was recorded with BA 3.0 mg/l+ IAA 1.5 mg/l in minimum 12.20 days and BA 2.0 mg/l+ IAA 1.0 mg/l gave the highest number of root 2.40, 3.20 and 4.20 at 14, 21 and 28 DAI, respectively. The survival rate was 80% in shade condition which was 75% in open atmospheric condition. Therefore, an efficient protocol has been developed for in vitro regeneration of chrysanthemum which has great commercial value for year round production in Bangladesh.