ANTIDIABETOGENIC IMPACT OF BITTER MELON (MOMORDICACHARANTIA) AND GARLIC (ALLIUM SATIVUM) ON ALLOXAN INDUCED DIABETIC RABBIT MODEL A Thesis By AMBIARA

Abstract

The present study was undertaken to investigate the antidiabetic effect of the bitter melon and garlic on alloxan induced diabetes in experimental rabbits. After acclimatization of the age of 4 months rabbits (New Zealand white) were randomly assigned into five groups (A, B, C, D and E) and each group was remained 4 rabbits. Group A was kept for negative control (without alloxan), Group B was kept for positive control (with alloxan) treated with alloxan intramuscullarly @75mg /kg body weight, Group C was treated with bitter melon @250mg/kg body weight orally along with alloxan, Group D was treated with garlic @750mg/kg body weight orally along with alloxan, Group E treated with combined at previous doses of bitter melon and garlic along with alloxan. Over the course of the trial, observations were recorded for induction of diabetics, blood glucose level, body weight, packed cell volume, ESR and Hb (Hemoglobin) level after 72 hours of injection. Blood glucose level were increased significantly (p<0.05) in all treated groups compared to the negative control group and the highest induction was recorded in group B treated with alloxan. Body weight was decreased significantly (p<0.05) in all treated groups and lowest was recorded in group B which received treatment Alloxan. There was significant decreased in blood glucose level in all treated group C, D, E compared to the B group and lowest glucose level was recorded in E groupwhen treated with combined medicinal herbs and body weight was increased in all treated group C, D, E compared to the B group and highest was recorded in D group. PCV level was the highest in group E which was treated with both garlic and bitter melon compare to the A group. The Hb ( Hemoglobin) gm/dl concentration was the highest in group E which was treated with both garlic and bitter melon compare to the A group. The study was revealed that the Hb (Hemoglobin) gm/dl concentration was the highest in group B treated with alloxan.ESR was highest in group B treated with alloxan and lowest in group E. The present study reveals that combined II treatment with bitter melon and garlic increases body weight and decreases glucose level without affecting health of rabbits. The results of this study show that chronic oral administration of a suspension of bitter melon and garlic is an appropriate dosage may be good alternative as antidiabetic agent.