ELISA-Based Screening of Tomato Varieties against Tomato Yellow Leaf Curl Virus (TYLCV)

Abstract

A pot experiment was conducted in net house under the Department of Plant Pathology, Sher-e-Bangla Agricultural University, Dhaka-1207, Bangladesh. The purpose of the study was to evaluate the incidence and severity level of the Tomato Yellow Leaf Curl Virus (TYLCV) and to screen the resistance/tolerance of selected tomato varieties against TYLCV through serological test (DASELISA).

DAS-ELISA test was performed at tissue culture laboratory of Bangladesh Council of Scientific and Industrial Research (BCSIR).The experiment was carried out during the period October 2017 to April 2018. In total ten tomato varieties viz. BARI Tomato-2, BARI Tomato-8, BARI Tomato-11, BARI Tomato-14, BARI Tomato-15, BARI Hybrid Tomato-5, BARI Hybrid Tomato-7, BARI Hybrid Tomato-9, Ratan and Sorno Komol were selected to conduct this study. Among the selected ten tomato varieties, the highest disease incidence (100%) was recorded in 5 (five) varieties viz. BARI Tomato-11, BARI Tomato-14, BARI Tomato-15, BARI Hybrid Tomato7

and Sorno Komol and there was no incidence was found in BARI Hybrid Tomato-5. The highest disease severity was recorded in BARI Hybrid Tomato7

(70%) and there was no severity was found in BARI Hybrid Tomato-5. Although the variety BARI Hybrid Tomato-5 was not shown any kind of typical symptoms in the net house conditions when investigated on the basis of biological properties but it was found infected in DAS-ELISA test. From the above mentioned results, it can be concluded that symptomology is not the reliable method for all virus identification/detection. Different growth parameters, growth and yield attributes were also studied in this piece of research work and it was revealed that morphology, physiology, growth and yield contributing factors are significantly affected by TYLCV infection in different varieties.