IN VITRO REGENERATION OF ORCHID (Dendrobium sp.)

Abstract

The experiment was conducted at the Biotechnology Laboratory of Department of Biotechnology, Sher-e-Bangla Agricultural University, Dhaka from January 2021 to June 2021 to investigate the effect of different plant growth hormone for shoot proliferation and root regeneration of Dendrobium sp. where shoot tips were used as explant. The experimental data were taken on different parameters of shoot and root growth. Different doses of individual shooting hormone(BA), individual rooting hormone(NAA) and the combination of these two hormones were experimented here. The minimum days to shoot induction were recorded 15 days from treatment T 8 (2.0 mg/L BA + 1.50 mg/L NAA). Maximum percentage (73.4%) of shoot induction was noticed in treatment T 8 (2.0 mg/L BA + 1.50 mg/L NAA) .The maximum days to root induction was 44 days and it was recorded in control treatment .The minimum days to root induction was 17 and it was observed from treatment T 8 (2.0 mg/L BA + 1.50 mg/L NAA) days. In the following study significant variation was observed for the use of BA and NAA on the percent of shoot induction explants. Significant variation was observed for the use of BA and NAA on the percent of root induction of explants . The treatment of 2.0 mg/L BA response excellent in shoot induction and 1.0 mg/L NAA showed better performance in root development in Dendrobium sp. Combined effect of BA and NAA was very significant. Overall a reliable protocol was developed for shoot and root proliferation which can be used for further crop improvement program.