Effects Of horizontal gene transfer on bacteriophage infectivity

Abstract

Vibrio cholerae is one of the very well-known and common pathogenic bacteria which cause mild to severe gastrointestinal diseases, and in extreme cases, patients die. Specially children are victims where proper clinical,environmental, and public health support are insufficient. As a treatment, antibiotics were promising until vibrio cholerae became multidrug resistant by raising a global concern. In our environment many antibiotic resistant genes are increasing day by day. Following previous successful studies, bacteriophage therapy for antibiotic resistant vibrio strains is considered as one of the potential solutions to such problems. In this study, six native strains of vibrio cholerae from the environment underwent antibiotic resistant transformation. The main purpose of this study is to observe the infectivity of selected bacteriophages on strains of similar genetic makeup following antibiotic resistant traits, especially ampicillin and kanamycin antibiotics. In other words, to find the potentiality of phage therapy. A positive finding will be beneficial for vibrio cholera treatment and contribute to clinical, environmental biotechnology and public health as well. Therefore, by isolating antibiotic resistant genes containing DNA, transforming those into native vibrio strains and finally double layer spot test on the prepared strains gave a conclusive and intriguing outcome of phage infectivity which favors future studies with more antibiotic resistant vibrio strains and bacteriophages.