Molecular Diagnosis of Brucella abortus in cattle

Abstract

One of the world’s most important zoonotic diseases like brucellosis, affected both animals and human robustly. In Bangladesh, late abortion commonly explored in dairy cattle by diverse types of causal agents where Brucella abortus is one of the major causal agents. In the study, this organism was isolated and identified through molecular characterization from aborted fetus of dairy cattle first time ever in Bangladesh. For the study, 25 aborted fetuses were collected and tested from 25 sero-positive dairy farm of Chittagong district. A total of 20% samples were found positive as Brucella abortus through culture and conventional PCR technique which is an authentication for the infectious agent. The organism varies from organ to organ likely eyeball (20%), liver (16%), lung (12%) and kidney (08%) according to sensitive and specific location. These findings were considered to be more useful in prevention and controlling against Brucellosis at Chittagong as well as in Bangladesh.