Isolation of bacteriophage using E. Coli ATCC-25922 and E3 strain from water samples around Bangladesh

Abstract

Bacteriophages are the most abundant organism on the Earth having simple structure but diverse characteristics. Since their discovery in 1915 till now they have proved to be useful to human as phage therapy, diagnostic weapon, genetic screening tool, detector of pathogenic bacteria, therapeutic agent and so on. With the recent increase of antibiotic resistance, phage research has attracted interest because of their ability to infect and kill bacteria without causing any harm to human. This experiment is based on the phage collection or isolation from environmental samples around Bangladesh to find out any presence of phages using two types of bacterial strains where one is the standard of the other. It contains protocols that have been tried in the laboratory to see the presence of phages and the outcomes from the experiments. The aim of this project is to detect any presence of phage in environment samples of Bangladesh.