ISOLATION OF Salmonella & E. coli FROM EGGSHELL SURFACE OF BACKYARD DUCKS

Abstract

This study was conducted to isolate Salmonella and E. coli from eggshell surface of ducks that reared in the backyard farming and determine the prevalence of detection in various area, breed and ages. A total number of forty samples were collected from upazila area of Sylhet division and examined over a period of three months. The entire samples were at first pre-enriched by using nutrient broth medium. Salmonella organisms were isolated in the Salmonella Shigella (SS) agar, Brilliant Green Agar (BGA) and finally identify through biochemical test in the Triple Sugar Iron medium. On the other hand E. coli was isolated through using MacConkey and Eosin Methylene Blue (EMB) agar. Out of this forty samples 3 (7.5%) were Salmonella positive and E. coli were found in 13 (33%) samples. High prevalence of Salmonella and E. coli was found in Nobigonj (20%) and in Bahubal (44%) respectively. Among the five breeds from which samples were collected the Pati ducks (The spot-billed ducks) shows high prevalence in both Salmonella (20%) and E. coli (20%) cases. The median age of both Salmonella and E. coli positive ducks are little bit higher than the age of ducks which were Salmonella and E. coli negative. Results also reveal that the prevalence of Salmonella and E. coli is higher among the diseased (20% & 50%) and non-vaccinated (14% & 43%) ducks rather than the non-diseased (3% & 27%) and vaccinated (0% & 21%) ones.