Cytotoxic, Antimicrobial & Antioxidant Activity of Ethyl Acetate Fraction of Syzygium Samarangense Leaves Extract

Abstract

The plant Syzygium Samarangense has been used for the general promotion of health and longevity by Asian tribal. It is used as a traditional medicine. The aim of the present study was to evaluate the cytotoxicity activity, antimicrobial activity and antioxidant activity of ethyl acetate extract of Syzygium Samarangense. The powdered leaves of Syzygium Samarangense were extracted with methanol and then partitioned with n-hexane, dichloromethane and ethyl acetate consecutively. The ethyl acetate fraction was used to evaluate cytotoxic, antimicrobial and antioxidant activities. The cytoxic activity was measured by brine shrimp lethality bioassay. Ethyl acetate fraction showed cytotoxic activity with LC50 value 3.45µg/ml in brine shrimp lethality test. The antimicrobial activities of ethyl acetate solvent extract of Syzygium Samarangense plant were tested against the Gram-positive and Gram-negative bacterial strains by observing the zone of inhibition. The antimicrobial test was performed by Disc diffusion method. The crude ethyl acetate extract of Syzygium Samarangense. The fraction contained 160.48mgAAE/g of total phenolic content and 1.302mg AAE/g total flavonoid content. The results of study indicate the presence of cytotoxic, antimicrobial and antioxidant properties of ethyl acetate extract. The obtained results provide a support for the use of this plant in traditional medicine and its further investigation