EFFECT OF GENOTYPE AND GENOTYPE x MEDIA ON ANTHER CULTURE OF WHEAT (Triticum aestivums

Abstract

An investigation was carried out to study the effect of genotype and interaction between genotype and media on callus induction and plant regeneration of wheat by anther culture. Eight wheat genotypes were cultured on three liquid media viz. AM, AMS, and MS. The response of the genotypes Pavon, Gourav, Kheri, Bijoy and Barkat were found quite satisfactory for callus induction. The results of the experiment revealed a wide range of variations in callus induction as influenced by different genotypes. Only percent of callus induction 4 weeks after culture showed significant variation in media x genotype. The genotype Pavon was the best for callus induction followed by Gourav. The interaction between media and genotype showed significant variation tor callus induction percent after 4 and 8 weeks. The interaction effect of AMS) and Pavon showed better performance for percent of callus induction at different time intervals. No significant difference was found in media and genotype interaction. Higher percent of green plants regeneration was observed in Gourav fullowed by Bijoy and Pavon. Bijoy produced lower percent of albino plants. Pavon showed best performance for number of green plant callus". For callus induction trom anther and plant regeneration the genotypes Pavon and Gourav were found suitable.