RELATIVE EFFICIENCY OF OOCYTES COLLECTION TECHNIQUES AND EVALUATION FROM GOAT OVARIES

Abstract

The present study was carried out at the Department of Animal Nutrition, Genetics and Breeding, Sher-e-Bangla Agricultural University, Dhaka during the period from January 2019 to December 2019. Goat ovaries were collected from Slaughter house of Dhaka city. After necessary processing the ovaries were categorized as right, left, ovaries without corpus luteum (CL), ovaries with corpus luteum (CL). Ovaries were then evaluated on the basis of length (cm), width (cm), weight (g), total number of follicles on the surface of each category ovaries, number of follicles aspirated, total number of cumulus-oocyte-complexes (COCs), normal and abnormal COCs. The length (cm) of right ovaries (1.31±0.04) was found significantly (p<0.05) higher than the left ones (1.18±0.04). The number of normal COCs (Grade A and Grade B) were found significantly (p<0.01) higher in left ovaries (2.14±0.08 and 1.65±0.08) then right ovaries (0.36±0.08 and 0.23±0.08) respectively. The number of abnormal COCs (Grade C and Grade D) were found significantly (p<0.01) higher in right ovaries (1.67±0.09 and 2.20±0.08) than left ovaries (0.33±0.09 and 0.34±0.08) respectively. Other parameters, including width, weight, total number of COCs did not differ significantly (p<0.05) between right and left ovaries. When compared between the ovaries with CL and without CL group significantly (p<0.01) higher number of normal COCs (Grade A and Grade B) were found without CL group (1.21±0.07 and 0.90±0.07) then with CL group (0.32±0.09 and 0.29±0.09) respectively. The number of abnormal COCs (Grade C and Grade D) were found significantly (p<0.01) higher in with CL group (0.82±0.09 and 1.18±0.09) then without CL group (0.31±0.06 and 0.39±0.07) respectively). Total number of follicles were found significantly (p<0.01) higher in without CL group (6.79±0.29) then with CL (5.84±0.21) group and number of follicles aspirated were found significantly (p<0.05) higher in without CL group (3.79±0.14) then with CL group (3.62±0.20). An increase of length (1.47±0.09) and total number of COCs (2.81±0.11) were found in without CL group, but decrease of length (1.34±0.14) and total number of COCs (2.62±0.16) in with CL group. An increase of width (0.74±0.05) in with CL group then without CL group (0.72±0.04). When COCs per ovary was compared among the collection techniques significantly (p<0.01) higher number of total COCs per ovary was yielded by slicing (22.20±2.40), followed by puncture (13.20±1.86) and aspiration (10.37±1.86). The number of normal COCs (Grade A and Grade B)were found significantly (p<0.05) higher in aspiration (3.80±0.60 and 3.33±0.42) followed by slicing (3.67±0.78 and 3.20±0.54) and puncture (2.20±0.60 and 1.40±0.42) respectively. The number of abnormal COCs (Grade C and Grade D) were found significantly (p<0.01) higher in slicing (7.00±1.02 and 8.33±0.98) followed by puncture (5.00±0.79 and 4.60±0.76) and aspiration (1.40±0.79 and 1.80±0.76).The maximum percent yield of normal COCs were higher in aspiration followed by slicing and puncture techniques. On the other hand, left ovaries contain more normal COCs and higher number of follicles than right ovaries; without CL ovaries contain higher number of follicles and normal COCs than with CL ovaries. Finally, it can be concluded that higher number of normal COCs found in left ovaries, without CL ovaries and aspiration technique is better for quality COCs.