PLANT REGENERATION PROTOCOL IN KENAF (Hibiscus canubinus L.)

Abstract

An experiment was conducted during the period of July 2006 to May 2007 in the Genetic Engineering Laboratory, Genetic Resources and Seed Division, Bangladesh Jute Research Institute, Dhaka, Bangladesh.. Kcnaf variety I-IC-2 was used to investigate the in vi'ro regeneration potentiality. Different concentrations and combinations of hormones, vitamins, FeSO.1 and surfactant (F-68) were supplemented to MS medium to observe callus induction and regeneration ability of kenaf. In cottonsupported MS liquid medium, the germination percentage was higher (91.00%) compared to agar supported MS medium (82.34%). Eight days old seedlings were highly responsive to shoot regeneration. Among the phylohormonc combination BA!' (3.0 mg/I) and IAA(0.5 mg/I) with MS supplemented showed the highest shoot regeneration. In different concentrations of vitamin, the highest percentage of shoot regeneration was obtained (81.25%) at 4.0 mg/I vitamin concentration. Among different concentrations of surfactant, the highest percentage was observed (81.00%) in 0.5% surfactant concentration. Using different concentration of' FeSO4 , the highest percentage of shoot regeneration was (9 1.67%) at 2.0 mg/I PeSO4 concentration. In present study, an efficient protocol for the plant regeneration has been developed which could be further used for genestic transformation in kenaf.