Investigation of in vitro antioxidant and cytotoxic potential of Triphala methanol extract in different ratios

Abstract

Triphala is comprised of Bibhitaki Amalaki and Haritaki and its methanol extract was chosen for the In vitro antioxidant and cytotoxic potential studies. Firstly, in the DPPH test the IC50 value was found to be 15.93 μg/ mL, 12.99 μg/ mL and 10.287 μg/ mL for Haritaki, Bibhitaki and Amlaki, and the IC50 value for Ascorbic Acid was 12.96 μg/ mL, so Amlaki showed more inhibition. The IC50 value for the Triphala ratios 1:1:1 (A:B:H), 2:1:1 (A:B:H), 1:2:1 (A:B:H) and 1:1:2 (A:B:H) were 11.28 μg/ mL, 7.01 μg/ mL, 8.33 μg/ mL and 11.03 μg/ mL respectively and all the values were lower than that of the standard. Secondly, at 1200 μg/ mL, the total phenolic content was found to be 368.94 mg, 395.09 mg and 260.65 mg for Bibhitaki, Amlaki and Haritaki respectively. Finally, at 1200 μg/ mL, the total antioxidant capacity was found to be 245.32mg, 485.5mg and 559.76mg for Bibhitaki, Amlaki and Haritaki respectively. In N4X4 cell line, IC50 value was 13.43mg/ mL and 13.23mg/ mL at 25 mg/ mL for 1:1:1(A:B:H) and 2:1:1 (A:B:H) Triphala ratio. To conclude, it can be inferred that the extracts showed strong antioxidant and moderate cytotoxic potential.