IN VITRO PROPAGATION OF BANANA (Musa spp.)

Abstract

The experiment was conducted at the Biotechnology Laboratory, Department of Biotechnology. Sher-e-Bangla Agricultural University. Dhaka, during October 2013 to August 2014 to investigate effects of hcnzylaminopurine (RAP) concentrations (1.0. 10, 3.0. 4.0 and 5.0 mg/L) and indole-3-butyric acid (IDA) (0.5. 1.0. 1.5. 2.0 and 2.5 mg/L) for in vitro regeneration of Sagar and Sabri Banana varieties using shoot tip explants. The highest response of explants 88% in Sagar and 84% in Sabri were with 4.0 and 5.0 mgfL BAP. whereas in 4.0 mg/L BAP+2.0 mg/L IRA had 85% response in Sagar and 5.0 mg/L BAP+2.5 mg/L IBA had 90% response in Sabri. In both Sagar and Sabri. minimum number of days for shoot initiation (7.80 and 9.40 days), maximum number of shoots per explant (6.80 and 3.40), highest shoot length (8.31 and 6.19 cm), number of leaf (4.24 and 4.49) were observed in 4.0 mg/L and 5.0 mg/L RAP. In contrast, due to combined effect, the highest shoot number per explant (5.60 and 3.40) was in 4.0 mg/L BAP+1 .5 mg/I. IRA and 5.0 mg/L I3AP+2.0 mg/L IDA in Sagar and Sabri. Again, root formation required minimum 7.60 and 8.00 days in 1.5 mg/L IRA for Sagar and Sabri, respectively. The highest number of roots (5.20 per explant) was observed in 0.5 mg/L of IRA for Sagar and that for Sabri was 3.40 per explants observed in 1.5 mg/L of IRA at 6 WAI. In combination both for Sagar and Sabri, the highest number of roots (5.00 and 5.20 per explant) and root length (6.61 and 6.72 cm) were produced by 3.0 mg/L BAP±1.0 mg/I. [BA. In natural environment. Sagar variety survived more (94.74%) in soil than Sabri variety (88.89%). Ultimately combined effect of RAP and IRA seemed better than individuals based on average performance of growth parameters and the protocol optimized in the present study could be used in vitro rapid propagation of Banana plantlets. iv