Isolation and identification of Klebsiella bacteriophages from surface water samples

Abstract

Bacteriophages to the antibiotic resistant bacteria have a broad application in the field of medical science as alternative to the conventional antibiotics. In this research the goal was to isolate and identify the bacteriophages against Klebsiella pneumoniae and reference Klebsiella pneumonia that are resistant to antibiotics. A series of techniques and protocols were used to get best possible outcome. Several water samples were collected with robust coverage from different ponds and lakes around Bangladesh in order to isolate the bacteriophages. Isolated bacteriophages were applied on the hard agar medium along with soft agar and respective bacterial strains to observe the plaques as bacteriophages. This research may help to reduce the cost, dependency on antibiotics and to kill the bacteria those are resistant to antibiotics as the world is facing a great challenge to control the resistant bacteria from newborn to older individuals.