ISOLATION, MOLECULAR IDENTIFICATION AND SCREENING OF FUSARIUM MYCOTOXIN DON (DEOXYNIV ALENOL) DEGRADING BACTERIA IN EUROPEAN WHEAT SAMPLES

Abstract

Mycotoxins are the toxic substances which are produced as a result of infection caused by fungi as their secondary metabolite. Major toxin producing fungi are Fusarium, Penicillium, Aspergillus and Claviceps which produces DON, ZEN, OTA, FB, AFB, citrinin and patulin. The experiment was carried out in Key Laboratory of Food Safety and Quality Control, Institute of Food Science and Technology.Chinese Academy of Agricultural Sciences, Beijing China under completely randomized design with three replications. The objectives of the experiment were the isolation, molecular identification and screening of the single bacterial colony which is responsible for the degradation of Fusarium mycotoxin deoxynivalenol (DON) in European wheat samples. Wheat samples were mixed using lysogeny broth (LB) media, PBS media and mineral salt (MM) media for isolation of bacteria using DONstar immunoaffinity column.Six Bacteria genera namely Pseudomonas spp, Agrobacterium sp, Zobellella sp, Bacillus sp., Achromobacter sp, Advenella sp and Cupriavidus sp. were isolated and identified by peR amplifying 16SrDNA gene fragment.Maximum DON degradation was recorded by mixed bacteria in wheat samples rather than the isolated single bacteria.