MICROPROPAGATION OF STRAWBERRY (Fragaria×ananassa Duch.)

Abstract

The present research was carried out in Biotechnology Laboratory of the Department of Biotechnology, Sher-e-Bangla Agricultural University, Sher-eBangla Nagar, Dhaka-1207 from the period of May 2018 to November 2018 for

in vitro renegaration of strawberry. The healthy, disease free shoot tips of 0.501.0

cm length were used as explants, It was sterilized by 0.1% HgCl 2 mixing with few drops Tween-20. The explant was inoculated in MS media supplemented with different combination of the BA (1.0, 2.0, 3.0, 4.0 and 5.0 mg/l) and IBA (0.50, 1.00, 1.50, 2.00 and 2.50 mg/l) either alone or in combination. The maximum days for shoot induction were recorded in control treatment. The highest number of shoots (3.60) in BA 2.0 mg/l and leaves (9.60) per explant observed in BA 3.0mg/l. In contrast combine treatment of 2.0 mg/l BA+1.00 mg/l IBA performed best in case of percentage shoot induction (80%) and number of shoots (3.60).The highest length of shoots (3.66 cm) in BA 2.0 mg/l+ IBA 2.0mg/l . It was noticed that the minimum days to shoot induction, percent shoot initiation and no. of shoot were recorded in 2.0 mg/l BA+1.0 mg/l IBA. The highest number of leaves per explant was recorded in 2.0 mg/l BA+0.5 mg/l IBA. The highest number of root was observed in 2.0 mg/l BA+ 1.5 mg/l IBA and the highest percent root initiation in 3.0 mg/l BA+1.5 mg/l IBA Overall, micropropagation was found to be very effective and promising method in the proliferation of strawberry and this experiment can be a useful tool for breeding program of strawberry. Finally, the survival rate of micropropagated plantlets were observed in open atmosphere 75% and 80% in controlled condition.