Investigatiin of antiatherogenic and antioxidative effects of aspirin in human diabetics

Abstract

Red cell reactive oxygen species scavengers namely reduced glutathione (GSM), superoxide dismutase (SOD) were significantly reduced in both type I and type II diabetic subjects compared to normal controls. The reductions were significantly less in subjects under aspirin therapy irrespective of age and sex of the diabetics indicating a significant restorative effect of aspirin. Of the restorative effects of aspirin that were observed, the most significant and impressive effect was on the red cell antioxidative enzyme glucose-6-phosphate dehydrogenase. The level of the enzyme was reduced by 35.1% and 25.9% in the female IDDM and NIDDM subjects respectively belonging to the age group of 20-40 years. The corresponding reductions were only 13.7% and 9.7% in the subjects under aspirin therapy. The restorative effects of aspirin were therefore 21.4% and 16.2%. Similar significant restorative effects of aspirin were observed in all subjects irrespective of age and sex. Significant restorative effect of aspirin was also seen on glutathione peroxidase. This was seen mainly in type I diabetics except the males of the 20-40 years age group. The restorative effect of aspirin was also observed on the other red cell antioxidative enzyme glutathione reductase. However this was observed only in males of the 20-40 years age group and females of 41-60 years age group. One of the most important lipid profile parameters that is measured to warn patients about atherosclerotic risk is serum LDC-C HDL-C ratio. The data presented in the tables 3.1, 3.3, 3.5, 3.7 are highly interesting. The ratio is significantly elevated in both type I (40.9%) and type II (18.5%) diabetic subjects of 41-60 years age group compared to normal controls. The ratio is found to be significantly depressed under aspirin therapy, which are 23.8% and 22.4% in type I and type II diabetics respectively. Similar effects of aspirin were observed in all subjects irrespective of age and sex except type II diabetics of 20-40 years age group. These results are certainly very interesting. The drug seems to significantly lower serum ratio which is consistent with its antiatherogenic effect. HDL-C Abstract Dhaka University Institutional Repository Content CHAPTER 1: INTRODUCTION PART - A l.A l.A.l l.A.2 l.A.3 l.A.4 1.A.5 1 .A.6 Diabetes mellitus Definition Symptoms of diabetes mellitus Classification of diabetes mellitus Diagnosis of diabetes mellitus Complications of diabetes mellitus Role of free radicals i.e. oxidative stress in the development of complications in diabetes a) Nature of collagen modification in diabetes b) Autooxidative glycosylation and glycoxidation in diabetes c) Lipid peroxidation in diabetes d) Oxidation of low density lipoprotein and atherosclerosis PART - B 1 .B ' Glutathione and oxyradical scavenging enzymes 1 .B. 1 1.B.2 Glutathione, the intracellular tripeptide and its functions a) Glutathione b) Functions of glutathione c) Glutathione and diabetes mellitus Glucose-6-phosphate dehydrogenase, the rate limiting enzyme of pentose phosphate pathway a) Glucose-6-phosphate dehydrogenase b) Functions of glucose-6-phosphate dehydrogenase