COMPARATIVE EFFICACY OF LENTOGENIC NEWCASTLE DISEASE VACCINES IN BROILER CHICKEN

Abstract

Newcastle disease is a devastating poultry disease all over the world including Bangladesh. The present study was conducted to investigate the comparative efficacy of ND lentogenic vaccines Cevac New L ® (Lasota strain), Avinew® (VG/GA strain) and BCRDV(F strain) in Broiler chicken during the period from January, 2014 to June, 2014 as well as isolation of Newcastle disease virus from clinical sample. For the purposes of this experiment 100 Broiler chicken were purchased from CP limited of Sylhet without history of vaccination against ND. The chickens were divided into 4 groups. In group A (n=25) and B (n=25), group C (n=25) vaccination was performed twice with BCRDV, Avinew® and Cevac New L at day 7 and 21 of age i/o, while group D (n=25) remained unvaccinated control. Serum antibody titre was evaluated by the means of hemagglutination inhibition test (HI) at 3 ®

and day (pre vaccination), 14 rd th day (post vaccination ) of age. It was observed that sera sample from selected birds revealed Mean±SD of HI titre 15.36±1.79, 89.60±5.70, 215.04±20.02, after 3 th day (post vaccination) and 28 days respectively for Group A and sera sample from selected birds revealed Mean±SD of HI titre 16.96±1.79, 97.28±5.70, 271.36±20.02 after 3 rd, th th 14 and 28 rd th days respectively for Group B. It was also observed that sera sample from selected birds revealed Mean±SD of HI titre 17.60±1.79, 101.12±5.70, th ,14 and 28 rd th 271.36±20.02 after 3 days respectively.The unvaccinated control Group D maintained a Mean±SD of HI test of 0.0±0 after 28 th ,14 and 28 day of post vaccination. This indicated that there were no Ab titres to protect against NDV (F strain) in respect of HI Ab titres response. For Isolation of virus, a total of 25 tracheal, lungs, proventriculus, and brain tissue were collected from Broiler chickens where chickens dying of classical respiratory, enteric and Nervous syndrome. The samples were inoculated to embryonated chicken egg via allantoic cavity route. The positive result were death of embryo ,HA(+), HI(+)for NDV.

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