IN VITRO PLANT REGENERATION OF THREE VARIETIES OF POTATO (Solanum tuberosum L.) FROM EPICOTYL

Abstract

The experiment was carried out during the period from December 2009 to April 2010, in the Tissue Culture Laboratory of the Department of Genetics and Plant Breeding, Hajee Mohammad Danesh Science and Technology University, Dinajpur. Callus induction, shoot formation, root induction and plantlet regeneration of three standard potato varieties viz. Cardinal, Diamant and Patrones from epicotyl of potato were carried out in this experiment. For callus induction, four levels of IAA (0.0, 0.1, 0.5 and 1.0 mg/L) and BAP (0.0, 1.0, 3.0 and 7.0 mg/L) and NAA (0.0, 0.01 and 0.1 mg/L) were used. Callus induction from epicotyl of potato was positively influenced by 0.5-1.0 mg/L IAA + 3.0-7.0 mg/L BAP, producing 100% calli within 6-12 days of culture. This treatment produced 27.72 - 53.50g callus with maximum growth rate of 133.75 mg/day. Plantlet regeneration markedly enhanced by BAP. All the three varieties showed better performance on plantlet regeneration with 1.0-2.0 mg/L BAP alone or 1.0 mg/L BAP + 0.01-0.1 mg/L NAA, producing maximum shoot per explant (2.01) and plant height (4.78 cm).