“Exploring the Phytochemical Profile and Therapeutic Potential of the Methanolic Extracts of Xantolis assamica leaves: An In-vivo & In-vitro Approaches”

Abstract

Background: Modern scientific inquiry consistently explores the medicinal potential of plants by analyzing their chemical composition and therapeutic effects. This study focuses on investigating the phytochemicals and healing properties of methanolic extracts from the leaves of Xantolis assamica, a plant native to Bangladesh and Assam. Objectives: The primary goals of this research were to analyze the phytochemical constituents of methanolic extracts using gas chromatography-mass spectrometry (GC-MS), assess thrombolytic activity, and evaluate hypoglycemic effects through an oral glucose tolerance test (OGTT) on Swiss Albino mice. Methods: After drying, X. assamica leaves underwent methanol extraction. GC-MS analysis identified various phytochemicals present. Thrombolytic activity was evaluated in vitro, and OGTT was conducted on Swiss Albino mice. Results: GC-MS analysis revealed diverse phytochemicals in the methanolic extract, including fatty acid esters, triterpenes, diterpenes, and steroids. The extract exhibited significant thrombolytic activity, with a clot dissolution rate of 32%. Additionally, OGTT demonstrated reduced blood glucose levels in mice treated with the extract compared to control and standard groups at 120 minutes. The plant extract, administered at a dosage of 250 mg/kg and 500 mg/kg body weight, resulted in blood glucose levels of 6.2±1.27 and 5.33±0.78 mmol/L, respectively. In comparison, the standard and control groups exhibited blood glucose values of 6.27±0.88 and 5.36±2.12 mmol/L, respectively. Conclusion: This study underscores the therapeutic potential of X. assamica leaves, particularly their thrombolytic and hypoglycemic effects. Further research is warranted to elucidate underlying mechanisms and explore the development of natural remedies.