Antibacterial efficacy of Lawsonia inermis against Escherichia coli of commercial broiler chicken

Abstract

Globe is gifted with worthy wealth of medicinal plant by almighty. The plant kingdom played an important role in survival of man by providing their products and active constituent. There is a far-reaching reliance that green medicines are healthier and more in noxious or more secured than synthetic one. The use of medicinal plant in pharmaceutical industry is going to be more popular due to inability to cause any side effect to patient and not creating any resistance to pathogenic organism where as many antibiotic are resistant to some harmful organism. Escherichia coli is a harmful Gram negative organism that cause colibacillosis in chicken and cause heavy economic losses in poultry farm. After collection of leave of Lawsonia inermis (henna) drying is done to make powder and then methanol as solvent is added to grinded leave. Finally extract was got by evaporating the methanol by rotary evaporator. Different concentrations were made by disk diffusion method the study was carried out. The methanolic extract of Lawsonia inermis leave showed a moderate sensitivity to this organism at 100mg/μL and create zone of inhibition measured 18mm in diameter. This medicinal plant has the antibacterial activities and act well against some bacteria like E coli. After doing experiment it can be recommended that the extract of henna can be used at certain concentration as a remedy of antibiotic resistance.