Cholinesterase Inhibitory Potential and Anti-oxidant Activities of Leaf and Bark Extract of Diospyros blancoi Relevant for the Treatment of Alzheimer’s Disease

Abstract

Oxidative stress and decreased level of neurotransmitter (especially acetylcholine) are main characteristics of Alzheimer’s disease (AD), a progressive neurodegenerative disease. Increasing the function of acetylcholine by inhibiting acetylcholinesterase or butyrylcholinesterase enzyme (both are responsible for the breakdown of Acetylcholine) and reducing oxidative stress with antioxidants are most effective treatment therapy of AD. Beside this atherothrombosis is a major cause of global life threatening cerebral diseases. Traditionally Diospyros blancoi has widely known for its medicinal values. Our aim is to investigate the cholinesterase inhibitory activities as well as thrombolytic activities of the bark and leaves crude methanolic extracts (CME) and other fractions in the treatment of AD and clotting disorder. The crude methanol extract was prepared by cold extraction method and were assessed for acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) inhibitory activity by the Ellman’s method. For thrombolytic activity clot lysis method were applied. The results revealed that the CME of both bark and leaf have Cholinesterase inhibitory activities as well as thrombolytic activities which may be useful in the treatment of