LOW COST MEDIA FOR IN VITRO REGENERATION OF Gynura procumbens (Lour.) Merr.

Abstract

The present study was undertaken at ‘‘

Horticultural Biotechnology and Stress Management Lab ’’ , Sher-e-Bangla Agricultural University, Dhaka.The single factor experiment was laid out in Complete Randomized Design with three replications. Nodal segment was used as explant and six types of different low cost medium (LCM) such as LCM-1 (100 ml coconut water); LCM-2 (150 ml coconut water); LCM-3 (200 ml coconut water); LCM-4 (100 ml banana pulp) ; LCM-5 (150 ml banana pulp); LCM-6 (200 ml banana pulp) and full strength of MS medium used as a control with three replication for standardization a low cost in vitro regeneration protocol for Gynura procumbens. All culture medium was supplemented with 2.0 mg/l BA + 0.5 mg/l NAA for shooting and 0.5 mg/l IBA was used for rooting. Culture media LCM-2 showed best performances in shoot initiation, shoot multiplication and root formation compared to other low cost culture medium. In terms of cost analysis, LCM-2 helps to reduce cost (78.22%) of the medium per plantlet as compare to the conventional MS medium. Acclimatization medium consisting garden soil + Vermicompost + cocodust (1:1:1, v/v) exhibited the best hardening medium with maximum survival rate (96.45%) and vigorous performances. Taken together, we can conclud that LCM-2 culture medium is a cost effective protocol and alternative to conventional MS medium for regeneration of Gynura procumbens aseptically.