MICROPROPAGATION OF Nicotiana tabacum

Abstract

The present research was carried out in Biotechnology Laboratory of the Department of Biotechnology, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka1207

from the period of september 2017 to June 2018 for in vitro renegaration of tobacco. The healthy, disease free shoot tips of 0.25-0.50 cm length were used as explants, It was sterilized by 0.1% HgCl mixing with few drops Tween-20. The explant was inoculated in MS media supplemented with different combination of the BA and IBA. The minimum days to shoot induction were achieved on BA 3.00 mg/L. The highest shoots and leaves per explant also observed in BA 3.00 mg/L. The combine effect of auxin and cytokinine hormone was also investigated. It was noticed that the minimum days to shoot induction were recorded in 3.00 mg/L BA+1.00 mg/L IBA. The highest number of shoot, number of leaf per explants and length of shoot and length of leaf were recorded in 3.00 mg/L BA+ 1.00 mg/L IBA. The highest number of root and length of root was observed in 3.00 mg/L BA+ 1.00 mg/L IBA. Micropropagation was found to be very effective and promising method in the proliferation of tobacco and this experiment can be a useful tool for tissue culture of tobacco 2