STUDY ON Escherichia coli COLIFORM MASTITIS OF DOE AND THEIR ANTIBIOGRAM

Abstract

Rearing of goat has an important impact in improving social-economic status of rural people of the country. Coliform mastitis caused by Escherichia coli is one the predominant constraints of this livestock production in this country. This study was designed to investigate the situation of coliform mastitis by E. coli in clinically affected goat in Chittagong. A total of 29 milk samples of mastitic doe were collected from upazilla veterinary hospital of Hathazari, Rangunia and SAQ Teaching Veterinary Hospital, CVASU during internship placements. Confirmation of coliform mastitis by E. coli was done following culture (on MacConkey and EMB agars), biochemical and staining techniques. Later, isolates were tested against 7 antimicrobials of 5 different groups using disc-diffusion technique. Epidemiological data were analyzed using STATA software to reveal their association with occurrence of E. coli coliform mastitis. Out of 29 samples 8 were confirmed as E. coli (prevalence 27.6%; 95% confidence interval (CI) 14.5%-45.9%). In relation to different host factors such as breed, age, body weight and parity the highest occurrence was observed in cross breed, doe of ≤ 2 years, doe having body weight of > 30 kg, and in doe of 1st parity which was 50%, (95% CI 2.5%-78.4%), 28.6% (95% CI 7.6-64.8), 36.4% (95% CI 15%- 64.8%), and 50% (95% CI 21.5%-78.45) respectively. E. coli isolates were highly sensitive to Gentamycin (75%) and Colisitn sulfate (75%) followed by Streptomycin (37.5%) and Ceftriaxone (37.5%). Highest resistance was recorded against Amoxicillin (100%) and Ciprofloxacin (100%) followed by Cefotaxime (62.5%), Ceftriaxone (50%). Statistical analysis using chi2 test showed that there is no significant relationship between host factors with the occurrence of E. coli coliform mastitis in doe. Study suggests use of Gentamycin and Colistin sulfate for the treatment of coliform mastitis in doe caused by E. coli.