ISOLATION, IDENTIFICATION AND ANTIBIOGRAM STUDY OF BACTERIA FROM DIARRHOEIC GOAT IN SPECIFIC AREA OF SYLHET

Abstract

The research work was conducted with 100 faecal samples collected directly from the rectum of diarrhoeic goats during the period of January/2014 to June/2014 of Sylhet govt. goat farm, sylhet, Govt.veterinary hospital, Mirjajangal Sylhet, Govt.veterinary hospital, jaintapur,Sylhet , Kajir bazar, Sylhet, Shah poran region, Sylhet to find out the causal agents of goat diarrhoea and antibiotic sensitivity against the goat diarrhoea. Isolation and identification of bacteria were confirmed on the basis of their morphology, staining, cultural and biochemical properties. Antibiotic sensitivity test was studied by disc diffusion method of Kirby-Bauer technique. Out of 100 faecal samples 76 samples (76%) were found positive for Escherichia coli, 80 samples (80%) for Salmonella spp. and 41 samples (41%) for Staphylococcus spp. Sensitivity test was conducted by 11 commonly used antibiotics with the test faecal samples. The results showed that out of 11 antibiotics, 5 antibiotics (Penicillin-G, Erythromycin, Amoxycillin, Bacitracin and Cefalexin) were resistant as these did not show any inhibition zone. Remaining 6 antibiotics were sensitive in which Ceftriaxone showed highest inhibitory zone about 34.8 mm in average of 5 samples. Cotrimoxazole showed 2

highest inhibitory zone about 34 mm in average of 5 samples. Gentamycin showed 3 nd highest inhibitory zone about 29.8 mm in average of 5 samples. The findings of the experiment speculate that the use of Ceftriaxone, Cotrimoxazole and Gentamycin might have the preference in clinical control of goat diarrhoea.This test provided the guideline for the veterinarian to select appropriate antibiotics to reduce the economic loss through selecting the sensitive antibiotics.