Bioseparation and Biochemical Activities of touch-menot Mimosa pudica l. (fabaceae) against Cryptolestes Pusillus and some harmful Microbes

Abstract

The present study illustrates the antimicrobial potency against eight bacterial and four fungal strains and the determination of MIC of crude extracts as well as purified compounds of Mimosa pudica L (Fabaceae). The antioxidant activity was observed through 2,2-Diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. The phytoconstituents of crude extracts of leaf, stem and root were isolated through column chromatography, thin layer chromatography (TLC) and GC-MS (Gas Chromatography- Mass Spectrometry) analyses. Insecticidal and repellent activity was tested on Cryptolestes pusillus. Three gram-positive bacterial strains viz. Bacillus cereus, Staphylococcus aureus, Bacillus zhanjiangensis and five gram-negative bacterial strains viz. Klebsiella pneumoniae, Shigella boydii, Shigella sonnei, Escherichia coli and Salmonella typhi were used for testing the antibacterial potency of petroleum ether, chloroform, ethyl acetate and methanol crude extracts of leaf, stem and root as well as isolated pure compounds of M. pudica and compared with standard antibiotic Ciprofloxacin (5μg/disc). The chloroform extract of root showed the highest potentiality among all the extracts of leaf, stem and root against all the bacterial strains where the zone of inhibition was 21.67±1.45mm against S. sonnei at 200μg/disc concentration. The susceptibility order of the extracts was: root > leaf > stem. The antifungal activity was tested against four fungal strains viz. Rhizopus oryzae, Fusarium proliferatum, Aspergillus niger and Candida albicans with a standard Nystatin (50μg/disc). R. oryzae showed the highest (13mm in diam.) zone of inhibition against ethyl acetate extract at 200μg/disc concentration, and that of for F. proliferatum 12mm against ethyl acetate extract, for A. niger12mm against chloroform extract and for C. albicans 13mm against chloroform extract of root at the same concentration.-----