An In-Vitro Study to Investigate anti-inflammatory and thrombolytic activity of methanolic extract of blumea lacera leaves

Abstract

Blumea lacera is a genus flowering plant which is a valuable ayurvedic medicine for centuries. Phytochemical contents of Blumea lacera extract figured the presence of different biochemical components like alkaloids, steroids, terpenoids, flavonoids, glycosides, tannins and phenolic compounds. The aim of the current study was to investigate the anti-inflammatory and thrombolytic property of Blumea lacera by observing inhibition of cell lysis and percent clot lysis. Five different concentrations of Blumea lacera extract were used as a test drug and aspirin, streptokinase were used as a standard drug for two experiments. The study showed dose dependent manner of haemolysis inhibition and good clot lysis activity as well. The maximum membrane stabilization of extract was found 72.80% at 500 μg/mL conc. in heat induced process and 74.08% at 500 μg/mL conc. in hypotonicity induced process and 61.79% clot lysis by extracts were found. Blume lacera possess significant anti-inflammatory as well as thrombolytic activity. However, further investigation can be carried out on isolation of individual phytocomponents and haemolysis inhibition by in-vivo procedure.