Efficacy of Different Concentrations of Seed Extract of Carica Papaya Against Clinical Isolates of Entamoeba Histolytica

Abstract

Amoebiasis is a major public health problem throughout the world. Almost Ten percent people of the world suffer from amoebiasis. The causative protozoan parasite, Entamoeba histolytica, is a potent pathogen, responsible for substantial morbidity and mortality in developing countries. It occurs mostly in tropics and subtropics. Poverty, ignorance, overcrowding, poor sanitation and malnutrition favor transmission and increased disease burden. Prevalence varies country to country and within a country. The present study was carried out to find out the efficacy of Carica papaya seed extract against clinical isolates of Entamoeba histolytica. The clinical isolates of Entamoeba histolytica was collected from the stool and cultured in culture medium. The concentrations of Carica papya seed extract were 3.954mg/ml, 1.972mg/ml, 0.986mg/ml, 0.493mg/ml, 0.216mg/ml, 0.108mg/ml and 0.054mg/ml. The incubation periods were 24 hours and 48 hours with different concentrations of clinical isolates of Entamoeba histolytica. The presence of viable and non viable Entamoeba histolytica was counted in each concentration of Carica papaya seed extract after incubation. The results show that the Carica papaya seed extract of different concentrations (3.95mg/ml, 1.97mg/ml and 0.98mg/ml) are highly sensitive to the clinical isolates of Entamoeba histolytica. The rate of inhibition of Entamoeba histolytica was 100% after 48 hours of incubation. It can be concluded that seed extract of Carica papaya in different concentrations (3.95mg/ml, 1.97mg/ml and 0.98mg/ml) can inhibit the clinical isolates of Entamoeba histolytica.