COMPARATIVE PERFORMANCE OF AMMONIUM NITRATE (NH ) AND OTHER NITROGENOUS SALTS FOR IN VITRO REGENERATION OF POTATO (Solanum tuberosum L.)

Abstract

An experiment was undertaken at the Biotechnology Laboratory, Department of Biotechnology, Sher-e-Bangla Agricultural University (SAU), Dhaka, Bangladesh during the period of January, 2018 to January, 2019 to identify a new chemical for the substitute of explosive chemical Ammonium nitrate (NH 4 NO ) in MS media composition for in vitro regeneration of potato (Solanum tuberosum L.). One potato variety Asterix was used as an experimental materials. The new chemical was denoted as α-chemical for the privacy of the experimental findings. MS powder, MS liquid standard dose, different concentrations (1, 5, and 10 gmL 3 ) of α chemical which have twice dose of other component in stock solution-I for the preparation of tissue culture media were used as treatment. It was noticed that in vitro regeneration of potato has been successfully done with α-chemical in new composition of tissue culture media. The shoot length, node number, number of leaf, root length were highest in MS powder (T ) at 14, 21 and 28 days after inoculation (DAI). Although, in some parameters MS powder (T ) gave maximum result but it was statistically non-significant with 5gm of α- chemical/litre in stock solution-I having twice dose of other components in new media composition. The treatment 5gm of α-chemical/litre in stock solution- I having twice dose of other component showed average performance in all the traits under studied. MS powder, Liquid MS standard dose and 1 gmL 0 -1 in stock solution- I twice dose of α chemical showed comparatively similar result on most of the parameters under studied. The newly used α-chemical is cheap, nonexplosive

and environmental friendly which can be an alternative of destructive chemical Ammonium nitrate (NH 4 NO ) for tissue culture media preparation.