Upregulation profile of miR-92b-3p and miR-210 in pre and post operative colorectal cancer patients

Abstract

Colorectal cancer (CRC) is a major global health burden and remains a leading cause of cancer-related morbidity and mortality worldwide. Late clinical presentation, tumor heterogeneity, and the limitations of invasive screening strategies underscore the need for reliable, non-invasive molecular biomarkers for early detection, prognosis, and treatment monitoring. MicroRNAs (miRNAs), small non-coding RNA molecules involved in post-transcriptional gene regulation, have emerged as key modulators of colorectal carcinogenesis and promising clinical biomarkers. This study aimed to investigate the expression patterns of miR-92b-3p and miR-210 in colorectal cancer patients and to evaluate their potential utility as biomarkers for disease monitoring and personalized therapy. Paired pre-operative and post-operative 14 blood samples were analyzed to assess changes in miRNA expression through real time PCR system. The pre-operative and post-operative samples associated with tumor removal and therapeutic response. In addition, socio-demographic factors and lifestyle characteristics including age, sex, educational status, occupation, dietary habits, physical activity, and sunlight exposure, were systematically examined to explore their associations with miRNA expression profiles. Quantitative analysis revealed differential expression of both miR-92b-3p and miR-210 between pre-operative and post-operative CRC samples, suggesting their involvement in tumor progression and response to treatment. Colorectal cancer patients demonstrated significant up-regulation of miR-92b-3p and miR-210 in pre-operative samples compared with post-operative patients and healthy controls, indicating tumor-associated expression patterns. Surgical intervention resulted in increased CT values, reflecting reduced miRNA expression and suggesting responsiveness to tumor removal. Overall, the findings of this study suggest that miR-92b-3p and miR-210 hold significant promise as non-invasive biomarkers for colorectal cancer diagnosis, prognosis, and post-treatment surveillance. Their integration with clinical and socio-demographic data may contribute to improved risk stratification and the development of personalized therapeutic strategies for CRC management.