IN VITRO PLANTLET REGENERATION OF RUCOLA (Eruca sativa)

Abstract

The present experiment was carried out in the Laboratory of Biotechnology, Department of Biotechnology, Sher-e-Bangla Agricultural University,Dhaka-1207 during the period of February, 2016 to June, 2017 to study the effect of BA (1.0, 2.0, 3.0, 4.0 and 5.0 mg/l),IBA (1.0, 2.0, 3.0, 4.0 and 5.0 mg/l) and NAA (0.5, 1.0, 1.5, 2.0 mg/l) either alone or in combination for direct and indirect in vitro regeneration of Rucola. The cotyledonary nodes of Rucola were used as explants which were sterilized with 70% ethanol and 0.2% HgCl .The highest frequency of shoot (80.00%) was observed in MS medium augmented with BA 3.0 mg/l in minimum (6.40 days). Maximum number of shoots(2.60, 3.60 and 4.60)were obtainedat 14, 21 and 28 days after shoot induction (DAI), respectively in BA 4.0 mg/L where the control treatment gave the lowest result.In combined effect, BA 4.0 mg/l+ IBA 3.0 mg/lshowed the highest percentage of shoot (76.00%) in 6 days and the maximum number of shoot (2.40, 3.0 and 3.40) at 14, 21 and 28 DAI, respectively. Besides, the highest number of leaves (3.20, 7.20 and 11.00) andlength of leaves (2.60, 4.42 and 5.00 cm) were observed in BA 4.0 mg/l+ IBA 4.0 mg/l and BA 4.0 mg/l+ IBA 2.0 mg/l at 14, 21 and 28 DAI, respectively. For root regeneration, the best result was recorded with BA 4.0 mg/l +IBA 2.0 mg/l in case of percentage of root(68.00%) and maximum number of roots(3.60) in BA 4.0 mg/l+ IBA 4.0 mg/lwhere the lowest result observed in control. On the other hand, BA 4.0 mg/l+ NAA 1.0 mg/l gave the optimum results in case of frequency of callus (68.00%) and weight of callus (1.71 g, 2.15 g and 2.80 g) at 20 , 30 and 40 DAI, respectively. Further, minimum days for shoot induction (10.80 days) and the highest number of shoots (3.00) were obtained from callus with BA4.0 mg/l+ NAA 1.0 mg/l. Then, IBA 3.0 mg/l produced the highest percentage of root(72.00%) in 14.40 days.The maximum number of root(2.60, 3.60 and 4.60) and the highest length of root (1.26 cm, 2.32 cm and 3.16 cm) at 14, 21 and 28 DAI, respectively was recorded with IBA 4.0 mg/l.Finally, the survival rate of in vitro regenerated plantlets was recorded 75 % innatural condition. Overall, feasible protocol has been developed for in vitro rapid regeneration of Rucola having potential application in breeding programfor qualities improvement in commercial use.