Ovarian category, follicles and oocytes analysis of Goat ovaries in view of in vitro production of embryos

Abstract

The study was undertaken to evaluate the slaughter house goat ovaries, follicle and cumulus-oocytecomplexes (COCs) with the view of in vitro production of embryos. Goat ovaries were categorized as right, left, with corpus leutum (CL) and without CL group. Ovaries were then evaluated on the basis of weight (gm), length (cm), width (cm), total number of follicle on the surface of each categorized ovaries, number of follicle aspirated, total number of COCs, normal COCs and abnormal COCs. The length (cm) of right ovaries (1.19±0.09) was found significantly (p<0.05) higher than left ones (1.15±0.04). The number of normal oocyte was found significantly higher (p<0.05) in left ovaries (1.18±0.10) then right ovaries (1.11±0.09). Other parameters, including width, weight and total number of COCs aspirated per ovary did not differ significantly (P<0.05) between right and left ovaries. When compared the ovaries in between with-CL and without-CL group, significantly (p<0.05) higher number of normal COCs (1.12±0.07) were found in without-CL group with an increase of length (1.17±0.01), total number of follicles (5.21±0.03), number of aspirated follicles (2.74±0.12) and total number of COCs (1.99±0.14), but decrease in weight (0.66±0.03), width (0.76±0.02) and abnormal COCs (0.87±0.15) per ovary. Therefore, it could conclude that, both right and left ovaries is great source for large number of oocyte and without-CL ovaries is considered as a suitable source to supply quality oocyte for in vitro embryo production of goat.