MOLECULAR SCREENING OF TRICHODERMA ISOLATES

dc.contributor.author	Bajwa, Amna Ali, Rukhsana
dc.date.accessioned	2013-03-20T09:40:25Z
dc.date.accessioned	2019-05-29T05:10:01Z
dc.date.available	2013-03-20T09:40:25Z
dc.date.available	2019-05-29T05:10:01Z
dc.date.issued	2013-01
dc.description.abstract	The molecular study is based on genomic DNA therefore DNA must be extracted in a pure and uncontaminated form. In this study, genomic DNA of twenty four isolates of Trichoderma species (T. harzianum, T. hamatum, T. koningii and T. pseudokoningii) indicated band of ~15Kb on 0.8% agarose gel and quality was determined by obtaining absorbance ratio (260/280) in the range of 1.7-1.9. Restriction fragment length polymorphism (RFLP) analyses were performed by using two restriction endonulease enzymes i.e., BamHI and HindIII. The BamHI represented results in the range of 500bp-750bp. 18S rRNA gene targeting was further carried out through optimization in ribotyping analysis.
dc.identifier.other	http://dspace.daffodilvarsity.edu.bd:8080/handle/20.500.11948/871
dc.identifier.uri	http://hdl.handle.net/20.500.11948/871
dc.language.iso	en
dc.publisher	Daffodil International University
dc.source	DIU Institutional Repository
dc.subject	Trichoderma harzianum, T. hamatum, T. koningii, T. pseudokoningii RFLP, restriction endonuleases, 18S rRNA.
dc.title	MOLECULAR SCREENING OF TRICHODERMA ISOLATES
dc.type	Article

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