Purification, Characterization and Structure-Function Analysis of Proteins from Pota,Nogeton Nodosus Root Stocks

Abstract

Potamogeton nodosus rootstock contained ash, moisture considerably but significantly higher amount of protein. Rootstock contained smaller amount of lipid as compared to and seeds fruits. It contains carbohydrate such as reducing sugar, non reducing sugar and starch. The root stock oil was also characterized with respect to its physicochemical properties such as specific gravity, refractive index, smoke point, flash point, fire point, cloud point, solidification point, pour point, saponification value, sapanification equivalent acid value, % FFA, and iodine value. -- Six lectins were purified from the root stock of Potamogeton nodosus, locally name as Ghechu. The method was accomplished by gel filtration of 100% ammonium sulphate saturated fraction from the crude extract on Sephadex G-50 followed by ion-exchange chromatography on DEAE-cellulose and on CM-cellulose. The purity of lectins were judged by polyacrylamide slab gel electrophoresis. The molecular weight of PNL-1, PNL-2, PNL-3, PNL-4, PNL-5 and PNL-6 as determined by Sephadex G-150 and polyacrylamide slab gel electrophoresis method was estimated to be about 98,000, 91,000, 77500, 68,500; 62,500 and 38,500 and 97,500, 83,000; 77,000; 67,500; 62,000 and 38,100 respectively. -----