EVALUATION OF OOCYTES RECOVERED FROM OVARIES OF BLACK BENGAL GOAT UNDER DIFFERENT RETRIVAL METHODS

Abstract

Black Bengal goat is the national pride of Bangladesh. Ovary is the key female reproductive organ of all the vertebrates. With the aim to study the ovarian morphometry of Black Bengal goat, both right and left ovaries were collected from the slaughter houses. For each of the specimens, gross parameters such as right, left, corpus luteum (CL) present and absent group and evaluated on the basis of weight (g), length (cm), width (cm), follicles aspirated and number and state of cumulus-oocyte-complexes (COCs), normal COCs and abnormal COCs, the effect of collection techniques on cumulus oocyte complexes (COCs) of goat oocytes There is no significant differences were found in right and left ovaries. On the other hand significantly higher weight [(1.30±0.30) vs (0.98±0.17) g] and width [(1.08±0.24) vs (0.89±0.22) cm] were found in CL-present group than those of CL-absent group of ovaries. The recovery rates of grade A (31.11%) and B (22.41%) oocytes were higher than that of grade C (19.83%) and D (26.61%). It was observed that, the total number of COCs per ovary as well as the number of abnormal COCs/ovary were significantly higher (p<0.05) in slicing (3.28 and 1.44, respectively) and dissection (3.44 and 1.76, respectively) followed by aspiration (3.20 and 0.84, respectively) technique. In contrast, the number of normal COCs/ovary was significantly higher (p<0.01) in aspiration (2.36) followed by slicing (1.84) and puncture (1.68) techniques. Periods as 2, 4, 6 and 8 hours yield 78%, 69%, 60% and 53% oocytes counts of good, fair, poor and bad quality oocytes respectively. Yield of oocyte was highest in dissection technique compared with aspiration and slicing technique but maximum number of normal oocyte obtained from aspiration technique.