Non-human primates, Escherichia coli, Antibiogram

Abstract

The aim of this study was to determine the resistance of different antimicrobial agents to Escherichia coli isolated from nonhuman primates at a wildlife-human interface. Bacterial isolates from fecal samples of wild non-human primates at Bangabandhu Sheikh Mujib Safari Park, Cox's Bazar (Dulhazra Safari Park) were screened for the presence of Escherichia coli. A total of 13 samples were tested during March, 2017 to May, 2017 at Poultry Research and Training Centre, Chittagong Veterinary and Animal Sciences, where 12 (92.3%) samples were positive for E. coli in bacteriological tests. Isolated E. coli were tested for resistance with five different antimicrobial agents (Amoxicillin, Ampicillin, Ciprofloxacin, Sulfamethoxazole, Colistin sulphate) which were carried out by the Kirby-Bauer disc diffusion method as per recommendation of CLSI (Clinical and Laboratory Standards Institute) and efficacy of antibiotics was determined by measuring the diameter of the zone of inhibition that results from diffusion of the agent into the medium surrounding the disc. The E. coli were found 100% resistant to Amoxicillin, Ampicillin, Sulfamethoxazole. Conversely, 100% sensitivity was observed in Colistin sulphate followed by Ciprofloxacin (41.67%). All of the isolates showed multiple antimicrobial resistances in non – human primates may be a continuing threat to the effectiveness of antibiotic therapeutic agents. Therefore, it is important to monitor the occurrence of antimicrobial resistance in wild non-human primates at Bangabandhu Sheikh Mujib Safari Park, Cox's Bazar which is very essential for public health context.