EFFECT OF SUBSTRATE AMOUNT AND MOTHER CULTURE (INOCULA) ON YEILD ATTRIBUTES AND YEILD OF REISHI MUSHROOM

Abstract

The experiment was conducted at the laboratory and culture house of Mushroom Development and Extension Programme, Mushroom Development Institute, Sobhanbang, Savar, Dhaka during the period from March to August 2021 to study the effect of different substrate amount and mother culture (inocula) on yield and yield attributes of Reishi mushroom. The experiment consists of two factors viz.: factor A: different amount of substrates; B 1 = 250 g, B 2 ii = 500 g, B 3 = 750 g, B 4 = 1250 g, B 6 = 1500 g and factor B: Types of mother culture; T 1 = 1000 g, B 5 = Bamboo stick, T 2 = Sawdust, T 3 = Wheat grain. The experiment was laid out in two factors Completely Randomized Design with six replications. Data were gathered on several growth and yield parameters and it was discovered that the analyzed parameters varied significantly. In case of different substrate amount, yield contributing characters such as maximum number of fruiting body per packet (4.92), maximum single weight of fruiting body (3.18 g), longest length of stalk (2.50 cm), highest diameter of stalk (1.60 cm), diameter of pileus (6.92 cm), thickness of pileus (1.73 cm), biological yield (97.52 g), economic yield (88.24 g), dry yield (61.27 g) was observed from B 4 treatment. In case of mother culture (inocula), maximum number of fruiting body per packet (4.67), weight of individual fruiting body (3.12 g), length of stalk (2.27 cm), diameter of stalk (1.56 cm), thickness of pileus (1.42 cm), highest biological yield (84.74 g), highest economic yield (73.24) and highest dry yield (54.39 g) was recorded from T 2 treatment. In case of combined effect, maximum number of fruiting body (4.82), highest single weight of fruiting body (3.59 g), longest length of stalk (2.77 cm), diameter of stalk (1.83 cm), diameter of pileus (7.25 cm), thickness of pileus (1.72 cm), maximum biological yield (98.20 g), maximum economic yield (88.65 g) and maximum dry yield (62.35 g) was recorded from the combination of B 4 T 2 treatment. Among the treatments B 4 (1000 g) substrate with T 2 (Sawdust) treatment combination exhibited better growth and yield of Reishi mushroom.