GROSS AND HISTOLOGICAL STUDIES OF THE LEFT AND RIGHT EPIDIDYMIS OF INDIGENOUS BULLS (Bos indicus)

Abstract

The research was designed to study the gross anatomy including length, breadth, weight, size, shape and circumference and as well as the microscopic structures of the epididymis of bull. This study was conducted in the Department of Anatomy & Histology, Hajee Mohammed Danesh Science & Technology University (HSTU), Dinajpur from July to December-2013. Ten testicles from sexually matured indigenous bulls were collected from Bahadur Bazar abattoir located about twelve kilometers away from HSTU campus. The right and left testes were removed by making incision through the scrotal skin, fascia and the tunica vaginalis. The spermatic cords were severed by using the scalpel blade to incise through it. This was done to get the testes removed and after this was done, each testis was put in a labeled nylon. The intra-scrotal testes were placed in a well-insulated flask at warm condition (37° C) immediately the maintained animals were slaughtered. These were transferred to the laboratory immediately. For the gross study various parameters including weight of testis & epididymis ( WTE), weight of testis (WT), weight of epididymis (WE), length of testis (LT), length of epididymis (LE), breath of testis (BT), circumference of testis (CT), circumference of testis & epididymis (CTE). The lengths of right epididymides were higher than the lengths of left epididymides; this is contrary to the weight values, which was higher than the right. While weight of testes and weight of epididymis were negatively correlated indicated that as one value was increasing, the second value was decreasing. It was observed that the length of testes (LT) were negatively correlated (P=0.01) when compared with other testicular parameters like WTE, WT, WE, LE, BT and CTE. There was significant correlation (p=0.05) between WTE and WT, CT and CTE. For histological studies, tissue sample were excised from the epiddidymis, fixed in 10% formalin solution and embedded in paraffin. Cross sections (6 sm thickness) were stained with hematoxylin and eosin and evaluated the histological structures of the epididymal caput, corpus and cauda. The ductus epididymis was lined with a pseudostratified stereociliated columnar epithelium surrounded by a small amount of loose connective tissue and circular smooth muscle fibers. Few sperm were found in the initial segment, but a large mass of sperm aggregates were located in the cauda. It was concluded that the lengths of right epididymides were higher than the lengths of left epididymides but the weight values were higher than the right. The diameter of left epididymis was higher than the right for which more spermatozoa deposited in the left epididymis.