MOLECULAR CHARACTERIZATION OF Mycoplasma gallisepticum AND Mycoplasma synoviae FROM LAYER AND BREEDER CHICKEN IN BANGLADESH

Abstract

ABSTRACT

Mycoplasma is very important diseases in poultry. The economic value of Mycoplasma is very high in poultry industry. There are very few studies of Mycoplasma in Bangladesh. So it is an opportunity to identification of Mycoplasma gallicepticum and Mycoplasma synoviae and its prevalence area will be helpful for industry as well as the Nation. A total sum of 73 tracheal swab samples were collected from layer and breeder of Gazipur, Mymenshingh, Chattagram, Rangpur, Narshingdi and Keshoregong area. For molecular identification, PCR was done with 16S rRNA MG-13 and MG-14 primers and 16SrRNA MSL-1 and MSL-2 primers. From 73 of layer and breeder swab sample, 28 pure isolates of M. gallicepticum and M. synoviae were detected. Among the isolates, 24.65% were M. gallicepticum and 13.7% were M. synoviae. In this study, 18 isolates were positive for M. gallicepticum in which 9 isolates were from layer and 9 isolates were from breeder. Prevalence percentage of M. gallicepticum was 22% in both cases of layer and breeder. Out of 73 samples, 10 isolates were positive for M. synoviae, where 10 isolates were from layer but no isolate from breeder was found positive. Prevalence percentage of M. synoviae was 31% in case of layer chicken. Prevalence of M. gallisepticum was 9% in Gazipur, 33% in Mymensingh, 13% in Narshingdi 45% in Chattagram. 33% in Rangpur and 25% in Keshoregong area. On the other hand, prevalence of M. synoviae was 9% in Gazipur, 50% in Narshingdi, 42% in Keshoregong area but in Mymenshingh, Chattagram and Rangpur, no prevalence of M. synoviae was found.

Key words: Layer, Breeder, Mycoplasma synoviae, Mycoplasma gallicepticum