Identification of multi drug resistant bacteria from local fresh juice and industrially processed juice

Abstract

Fruit juices are widely consumed in many tropical nations and are known for their high nutritional value and mineral and vitamin content. In most cultures, fresh foods like fruits and vegetables are a staple of the average person's diet and are consumed in considerable quantities. The study aimed to detect the presence of pathogens in the fresh and packaged juices that the mass people in Bangladesh are consuming. The presence of Escherichia coli, Salmonella, Shigella, Klebsiella Pneumoniae selected from the family 'Enterobacteriaceae' and Listeria monocytogenes, Pseudomonas, was the main focus of the study. Among the collected samples, 4 were industrially packaged juices, and four were locally made fresh juices collected from Dhaka city. The samples were collected in sterile test tubes and subjected to total viable count (presence of the targeted organisms) by spread plate method. Later, an antibiogram test was done against ten commonly used antibiotics to determine the susceptibility of the targeted organisms. There were 129 isolates collected from these samples. Among 129 isolated colonies, some notable ones were Yersinia(28.0%), E.coli (8.0%), Enterobacter (6.0%), Salmonella spp (6.0%), Shigella(2.0%), Vibrio (4.0%). Among 50 selected antibiotic-resistant bacteria, 30 MDR were from the local fresh juices, and the rest 20 were collected from the packaged. This study shows that there are significant medical implications when microorganisms develop resistance to widely used antibiotics and medications. Our results confirmed the presence of pathogens in the juices, significantly deteriorating public health.