IN VITRO PROPAGATION OF TURMERIC (Curcuma longa L.)

Abstract

The present study was undertaken in Biotechnology Laboratory of the Department of Biotechnology, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka1207, during the period from February 2018 to June 2018 to evaluate the effect of different

plant

growth regulators (BA, IBA) on in vitro plantlet regeneration of Turmeric. The healthy, disease free sprouts of turmeric were used as explants which were inoculated in MS media supplemented with different conc. of Benzyl adenine (BA) (1.0, 2.0, 3.0 and 4.0 mg/L) and IBA (1.0, 2.0, 3.0 and 4.0 mg/L) either alone or in combination for shoot and root regeneration. The experiments were arranged in Randomized Completely Block Design (RCBD) with three replications. The maximum shoot induction was obtained with 2.0 mg/L BA. The highest shoot length (5.93 cm) was noticed in BA 2.0 mg/L + IBA 3.0 mg/L and minimum shoot length (1.4 cm) with control MS dose was observed. The highest percentage (81.00%) of shoot induction was recorded with BA 2.0 mg/L in minimum (14.0 days). Where, IBA 3.0 mg/L had produced the highest percentage of root induction (82.00%) required minimum (20 days). The highest length of shoot (3.4 cm, 5 cm and 5.9 cm) at 21, 28 and 42 DAI respectively was found in BA 2.0 mg/L + IBA 3.0 mg/L. The treatment BA 2.0 mg/L + IBA 1.0 mg/L gave the highest number of root (6.0, 8.6, 12.67) at 21, 28 and 42 DAI respectively and the highest length of root (2.7 cm, 4.8 cm and 5.4 cm) at 21, 28 and 42 DAI, respectively was found in BA 2.0 mg/L + IBA 1.0 mg/L. Regenerated plantlets showed, 100% survival during in growth chamber conditions and 86.66% in shade house stage of hardening and 83% in open atmosphere at direct sunlight. Therefore, an efficient protocol has been developed for in vitro propagation of Turmeric which has great commercial value for year round production in Bangladesh.