IDENTIFICATION OF A NEW CHEMICAL AS A SUBSTITUTE OF AMMONIUM NITRATE (NH 4 NO ) IN MS MEDIA PREPARATION FOR IN VITRO REGENERATION OF POTATO (Solanum tuberosum L.)

Abstract

An experiment was undertaken at the Biotechnology Laboratory, Department of Biotechnology, Sher-e-Bangla Agricultural University (SAU), Dhaka, Bangladesh during the period of January, 2015 to June, 2016 to identify a new chemical for the substitute of explosive chemical Ammonium nitrate (NH 4 NO ) in MS media composition for in vitro regeneration of potato (Solanum tuberosum L.). Three potato varieties viz Diamant, Cardinal and Asterix were used as an experimental materials. The new chemical was denoted as β chemical for the privacy of the experimental findings. Different concentrations (1, 5. 10, 15 and 20 gmL 3 -1 ) of β chemical were used for the preparation of stock solution- A, which was finally used for MS media preparation. It was noticed that in vitro regeneration of potato has been successfully done with new composition of β chemical in MS media preparation. The shoot length, node number, number of leaf, root length were highest in 5 gmL -1 of β chemical at 14, 21 and 30 days after sub-culture (DAS). Although, in some parameters liquid MS standard dose (16.5 gmL ) gave maximum result but it was statistically non significant with 5 gmL of β chemical. All the plantlets were died at higher concentration (15 gmL and 20 gmL -1 ) of β chemical. It may be due to toxic effect of the chemical. The variety Diamant showed best performance in all the traits under studied. It is reveal that ready made MS powder, Liquid MS standard dose and 1 gmL of β chemical showed comparatively similar result on most of the parameters under studied. Among the varieties, the Asterix variety showed minimal performance in most of the phenotypic characters under studied. In all the case, Diamant variety showed best performance for all the phenotypic characters under studied. The newly used β chemical is cheap, non explosive and environmental friendly which can be an alternative of destructive chemical Ammonium nitrate

-1 -1 -1 -1 (NH 4 NO ) for MS media preparation. 3