REGENERATION OF POTATO (SOLANUM TUBEROSUM L.) BY UTILIZING A NEW COMPOSITION OF STOCK SOLUTION-1 FOR PLANT TISSUE CULTURE MEDIUM

Abstract

An experiment was conducted at North Bengal Agro Farms Ltd., Thakurgaon, Bangladesh, during the period of January to September 2020 for plantlet regeneration of potato using different new composition of stock solution-1. Three chemicals viz. chemi-i, chemi-ii and chemi-iii were used in stock solution-1 as an alternate of ammonium nitrate. Eight different treatments were designed with those chemicals including Murashige and Skoog (1962) culture media as a check treatment (T ). Two potato varieties viz. Diamant and Asterix were used for in vitro regeneration. The Diamant variety showed the best regeneration potentiality in the T 3 , which consists of the chemi-iii. The check treatment (T 3 ) showed 2 nd 7 highest performance for all the parameters under studied. The Asterix variety gave the best performance in the check treatment (T 3 ) and the 2 nd highest result was found in the T . Different abnormalities were noticed in respect of media and plantlet regeneration. Brown, deep brown, off white and contaminated colour was found in the treatments T 4 1 , T 2 , T 5 , T 6 and T . Plantlet regeneration from those treatments showed aberrancy and it has stunted growth, dwarf size, small leaf formation, black layer at the root tips and finally plantlet died within 4 th week of regeneration. The T 7 8 treatment showed yellow colour and the plantlet regenerated from this treatment were thin, tall with less number of roots. The plantlet regenerated from check treatment (T ) showed good growth and development throughout the experimental period. Finally, it can be concluded that, the chemi-iii, can be used as a component of stock solution-1 which will be a good alternate of ammonium nitrate.