MOLECULAR DIVERSITY ANALYSIS OF AMAN RICE GERMPLASM USING SSR MARKERS

Abstract

An experiment was carried out at the laboratory of Genetic Resources and Seed Division (GRSD), Bangladesh Rice Research Institute (BRRI), Joydebpur, Gazipur, during the period of January 2020 to August 2020 for genetic diversity analysis within 18 Aman rice germplasm. Eleven rice genome specific SSR Primers were used for molecular diversity analysis. All of the eleven primers were capable to produce polymorphic bands. A maximum (6) number of bands was observed in SSR primers RM206. Thirty Eight alleles were detected with an average of 3.45 alleles. The Polymorphic Information Content (PIC) of SSR markers ranged from 0.10 (RM455) to 0.75 (RM206) with an average value of PIC = 0.42. Gene diversity ranges from 0.10 (RM455) to 0.78 (RM206) with a mean value of 0.45. The RM206 marker can be considered as the best marker among the studied markers for 18 Aman rice germplasm. Four primers showed high value of genetic diversity, five primers showed moderate diversity and two primers showed low diversity value on the basis of PIC value. Nei’s genetic distance (D) among 18 rice germplasm was computed from combined data for the 11 primers, ranged from 0.09 to 0.82 with an average of 0.46. Dendrogram based on Nei’s genetic distance using Unweighted Pair Group Method of Arithmetic Mean (UPGMA) indicated the segregation of 18 genotypes into five main clusters. This result could be further used for molecular diversity analysis and identify parent for the development of high yielding variety of rice.