GC-MS employed identification of phytoconstituents and antioxidant activity study of coriander leaf extract

Abstract

Coriander is a plant of the Apiaceae family and is very popular in herbal medicine world in and different cuisines. The study was conducted to identify the phytoconstituents that are present in the plant and investigate its antioxidant activity as other studies on the same plant showed therapeutic activities. GC-MS analysis was done in order to identify the phytoconstituents present in the sample and a total of 59 compounds were identified which have several therapeutic benefits. Based on the area % some of the major compounds present in the sample are 13-Docosenamide (Z)(49.82%), Decinamide(4.16%) Stigmasterol(3.45%), Tatetracinamide(3.33%) etc. and majority of these compounds had antioxidant activity. TPC, TFC and DPPH free radical scavenging assay was conducted to measure the antioxidant activity. The average TPC of three sample was 88.595 mg of GAE/g of dry extract at concentration of 88 mg/l of the extract. The average TFC of three sample was 184.5 mg/g RE of dry extract at concentrations of 234.634 mg/l of the extract. Lastly, average the % of inhibition of DPPH free radical by the sample extract was 64.1% at 8320.412 mg/l concentration. Literature review revealed that most of the identified compounds have antioxidant potential thus, it can be said that coriander can be used in treatment of the disease induced by free radicals as it has antioxidant properties.