Detecting Salmonella from Egg shell of Backyard Chicken.

Abstract

A study was conducted to determine the incidence of Salmonella spp. in egg shell surface and egg storing places of backyard chickens. A total of 45 egg shell surface and 35 egg storing places were examined in different areas of Comilla district, Bangladesh. Common culture methods used for the isolation of Salmonella from samples, nonselective pre-enrichment followed by selective enrichment and plating on selective and differential agars and expected colonies are then confirmed biochemically. Salmonella contamination was recorded in 6 of 80 samples (7.5%), of which 3 from egg shell surface (6.7%) and 3 from egg storing places (8.6%). Prevalence of Salmonella spp. in egg shell surface and egg containing place due to poor handling of eggs and reuse of egg storing places respectively. Salmonella contamination also recorded high in low temperature of January (15%) than high temperature of April (3.33%). Further examination of the Salmonella spp. was carried out by testing their antimicrobial sensitivity against 6 commonly used antibiotic in market. Among the antibiotics ciprofloxacin and gentamycin has the highest sensitivity and highest resistance was in tetracycline and ampicillin among the isolated sample.