IN VITRO REGENERATION OF GLADIOLUS (Gladiolus sp.)

Abstract

The present research was carried out in Biotechnology Laboratory of the Department of Biotechnology, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka -1207 from the period of July 2020 to June 2021 for in vitro rapid regeneration of Gladiolus. The healthy, disease free cormel of 1.5 cm- 2.0 cm were used as explants, was sterilized by 0.1% Bavistin followed by 0.1% HgCl2 mixing with few drops Tween-20. The explant were inoculated in MS media supplemented with different combination of Benzyl Adenene (1.0, 2.0, 3.0, 4.0, 5.0 mg/L) and Naphthalene Acetic Acid (0.5, 1.0, 1.5, 2.0 mg/L). The minimum days (11.00) to shoot (73.80%) induction with the highest percent of shoots induction were achieved in 4.0 mg/L BA. The highest number of shoot (1.60, 2.20, 2.40) and maximum shoot length (0.74 cm, 1.18 cm and 1.32 cm) were also observed in 4.0 mg/L BA and at 21, 35 and 45 Days After Induction (DAI) respectively. The combine effect of auxins and cytokinins were also investigated where 4.0 mg/L BA + 0.5 mg/L NAA performed the best incase highest frequency of shoot induction (74.80%) with minimum days (10.60) and gave the maximum shoot number (1.80, 2.60, 2.80) and shoot length (1.40 cm, 2.32cm, 2.82 cm) at 21, 35 and 42 DAI respectively. Moreover, the treatment 4.0 mg/L BA + 2.0 mg/L NAA showed the highest frequency (79.80%) of root induction with minimum days (8.40) and gave the maximum number (4.20, 6.00, 7.20) of root with optimum root length (1.02 cm, 1.26 cm, 1.34 cm) at 21, 35 and 42 DAI. In shaded condition the survival rate of plantlet was 83.33% and in open atmospheric condition the survival rate was 76%. Overall, a reliable and feasible protocol had been developed for in vitro multiplication of Gladiolus which can be a useful tool for tissue culture of Gladiolus