IN VITRO REGENERATION POTENTIALITY OF POTATO (So!anwn titherosum L.) CULTIVARS UNDER DIFFERENT CHEMICAL MUTAGENS

Abstract

The present study was undertaken in the Genetics and Plant Breeding Laboratory and the Tissue Culture Laboratory of the Department of Biotechnology. Sher-e- Bangla Agricultural University (SAU), Dhaka, during the period of January 2013 to March 2014 , to investigate the in vitro regeneration potentiality of three potato cultivars viz. Diamant, Cardinal. and Granola under different chemical mutagen treatment. Five tevels of GA3 (100, 200, 300,400 and 500 ppmIL) were used to find out the best concentration for sprout initiation . It was revealed that 500 ppmlL GA3 showed maximum sprouting within short period of time. The effect of concentration of 2,4-I) EMS and 5-RU on in vitro callus induction and plant regeneration were studied on I mg/L, 3mg/L, 5mg/Id concentration. Only 2, 4-D had the ability to induce callus. The highest callus size ( 2.30 cm) was observed at MS+3A0 mgfL 2,4-D with the variety Granola. The maximum number of shoot (13.67) were found in the treatment MS-F3.0 mgfL 2,4-D in the variety Granola at 45 DAI and the highest number of root (14.0) were also found in the same treatment. The highest number of shoot/plantlet (7.30) was recorded in variety Granola at MS + 1.0 mg/L EMS and the highest number of leaves/plantlet were also recorded in the same variety (17.30 leaves/plantlet) at the concentration of MS+3.0 mgfL at 45 DA!. The highest number of roots/plantlet was noted in the variety Granola (11.00 roots/plantlet) at normal MS medium at 45 DAL The higher concentration (5.0 mg/I) of EMS and RU showed huge abnormality on in vitro regeneration in all three varieties of potato. Thin stem, deformed shoot development and very less leaf formation were also observed in higher concentration. The mutagen treated variants were acclimatized in plastic tray and subsequently in the field condition. It was noticed that, the variety Granola survived at the high rate in plastic tray and in open atmosphere . Among all the varieties Granola showed the best performance on callus induction plant regeneration and in vivo establishment under field condition.The mutagenic treated minituber were harvested and preserved for further study.